"Cowboy Up" is a celebration of the risk-taking, daredevil personalities of modern rodeo. In the world of professional bull riding, newcomer Ely Braxton (Marcus Thomas) is the craziest guy around; his brother Hank (Kiefer Sutherland) is a rodeo clown, and the two use each other to play up their acts. But love may be one thing that tears the brothers apart. When Ely falls for the rodeo's sweetheart (Daryl Hannah), Hank is filled with jealousy and hatred. The brothers try to come to grips with their differences, but the competition gets as fierce as the bulls in the ring.
P'Sibtit, a bad boy, and charming cowboy from the Faculty of Agriculture. Prikkang is a down-to-earth girl who dislikes narcissistic and arrogant people so when she meets P'Sibtit she starts off the wrong foot with him. He is, on the other hand, quite smitten with her cold demeanor and tries to win her over. However, what they are not aware of is that they had used to be childhood friends before Prikkang moved from the countryside.